-
Depolymerization dynamics of individual filaments of bacterial cytoskeletal protein FtsZ
Proc Natl Acad Sci USA. 2012 May 22;109(21):8133-8 Mateos-Gil P, Paez A, Hörger I, Rivas G, Vicente M , Tarazona P, Vélez M. We report observation and analysis of the depolymerization filaments of the bacterial cytoskeletal protein FtsZ (filament...
-
Using DNA as a fiducial marker to study SMC complex interactions with the atomic force microscope
Biophys J. 2012 Feb 22;102(4):839-48 Fuentes-Perez ME, Gwynn EJ, Dillingham MS, Moreno-Herrero F . Atomic force microscopy can potentially provide information on protein volumes, shapes, and interactions but is susceptible to variable tip-induced...
-
Diacylglycerol kinase ζ controls diacylglycerol metabolism at the immunological synapse
Mol Biol Cell. 2011 Nov;22(22):4406-14 Gharbi SI, Rincón E, Avila-Flores A, Torres-Ayuso P, Almena M, Cobos MA, Albar JP , Mérida I . Diacylglycerol (DAG) generation at the T cell immunological synapse (IS) determines the correct activation of...
-
Monocytes as effector cells: activated Ly-6C(high) mouse monocytes migrate to the lymph nodes through the lymph and cross-present antigens to CD8(+) T cells
Eur J Immunol. 2012 May 15 Leirião P, Del Fresno C, Ardavín C . Monocytes have the capacity to differentiate into macrophages or dendritic cells (DCs) after extravasation into lymphoid and non-lymphoid tissues. They have thus been consequently...
-
Cryphonectria nitschkei virus 1 structure shows that the capsid protein of chrysoviruses is a duplicated helix-rich fold conserved in fungal double-stranded RNA viruses
J Virol. 2012 May 16 Gómez-Blanco J, Luque D, González JM, Carrascosa JL , Alfonso C, Trus B, Havens WM, Ghabrial SA, Castón JR . Cryo-electron microscopy reconstruction of Cryphonectria nitschkei virus 1, a dsRNA virus, shows that the capsid protein...
-
Regulation of translation initiation under abiotic stress conditions in plants: is it a conserved or not so conserved process among eukaryotes?
Comp Funct Genomics. 2012;2012:406357 Muñoz A, Castellano MM. For years, the study of gene expression regulation of plants in response to stress conditions has been focused mainly on the analysis of transcriptional changes. However, the knowledge on...
-
Structure of the receptor-binding carboxy-terminal domain of bacteriophage T7 tail fibers
Proc Natl Acad Sci USA. 2012 May 29 Garcia-Doval C, van Raaij MJ . The six bacteriophage T7 tail fibers, homo-trimers of gene product 17, are thought to be responsible for the first specific, albeit reversible, attachment to Escherichia coli...
-
Epitope insertion at the N-terminal molecular switch of the rabbit hemorrhagic disease virus T=3 capsid protein leads to larger T=4 capsids
J Virol. 2012 Apr 4 Luque D, González JM, Gómez-Blanco J, Marabini R, Chichón J, Mena I, Angulo I, Carrascosa JL, Verdaguer N, Trus BL, Bárcena J, Castón JR . Viruses need only one or a few structural capsid proteins to build an infectious particle....
-
Removal of vaccinia virus genes that block interferon type I and II pathways improves adaptive and memory responses of the HIV/AIDS vaccine candidate NYVAC-C in mice
J Virol. 2012 May;86(9):5026-38 Gómez CE, Perdiguero B, Nájera JL, Sorzano CO, Jiménez V, González-Sanz R, Esteban M . Poxviruses encode multiple inhibitors of the interferon (IFN) system, acting at different levels and blocking the induction of host...
-
The logic layout of the TOL network of Pseudomonas putida pWW0 plasmid stems from a metabolic amplifier motif (MAM) that optimizes biodegradation of m-xylene
BMC Syst Biol. 2011 Nov 11;5:191 Silva-Rocha R, de Jong H, Tamames J , de Lorenzo V . The genetic network of the TOL plasmid pWW0 of the soil bacterium Pseudomonas putida mt-2 for catabolism of m- xylene is an archetypal model for environmental...
-
Two small RNAs, CrcY and CrcZ, act in concert to sequester the Crc global regulator in Pseudomonas putida, modulating catabolite repression
Mol Microbiol. 2012 Jan;83(1):24-40 Moreno R, Fonseca P, Rojo F . The Crc protein is a translational repressor that recognizes a specific target at some mRNAs, controlling catabolite repression and co-ordinating carbon metabolism in pseudomonads. In...
-
Resolving structure and mechanical properties at the nanoscale of viruses with frequency modulation atomic force microscopy
PLoS ONE 2012;7(1):e30204 Martinez-Martin D, Carrasco C, Hernando-Perez M, de Pablo PJ, Gomez-Herrero J, Perez R, Mateu MG, Carrascosa JL , Kiracofe D, Melcher J, Raman A. Structural Biology (SB) techniques are particularly successful in solving virus...
-
The Escherichia coli SOS gene dinF protects against oxidative stress and bile salts
PLoS ONE Rodríguez-Beltrán J, Rodríguez-Rojas A, Guelfo JR, Couce A, Blázquez J . DNA is constantly damaged by physical and chemical factors, including reactive oxygen species (ROS), such as superoxide radical (O 2 − ), hydrogen peroxide (H 2 O 2 ) and...
-
Phytochrome interacting factors 4 and 5 control seedling growth in changing light conditions by directly controlling auxin signaling
Plant Journal Hornitschek P, Kohnen MV, Lorrain S, Rougemont J, Ljung K, López-Vidriero I, Franco-Zorrilla JM, Solano R , Trevisan M, Pradervand S, Xenarios I, Fankhauser C. Plant growth is strongly influenced by the presence of neighbors competing for...
-
Effect of Cry1Ab protein on rhizobacterial communities of Bt-maize over a four-year cultivation period
PLoS ONE Barriuso J, Valverde JR, Mellado RP . Bt-maize is a transgenic variety of maize expressing the Cry toxin from Bacillus turingiensis . The potential accumulation of the relative effect of the transgenic modification and the cry toxin on the...
-
Novel natural alleles at FLC and LVR loci account for enhanced vernalization responses in Arabidopsis thaliana
Plant, Cell & Environment Sánchez-Bermejo E, Méndez-Vigo B, Picó FX, Martínez-Zapater JM, Alonso-Blanco C . Vernalization, the induction of flowering by low winter temperatures, is likely to be involved in plant climatic adaptation. However, the...
-
Vaccine efficacy against Malaria by the combination of porcine parvovirus-like particles and vaccinia virus vectors expressing CS of Plasmodium
PLoS ONE Rodríguez D, González-Aseguinolaza G, Rodríguez JR, Vijayan A, Gherardi M, Rueda P, Casal JI, Esteban M . With the aim to develop an efficient and cost-effective approach to control malaria, we have generated porcine parvovirus-like particles...
-
Systems Analysis of MVA-C Induced Immune Response Reveals Its Significance as a Vaccine Candidate against HIV/AIDS of Clade C
PLoS ONE Gómez CE, Perdiguero1 B, Jiménez V, Filali-Mouhim A, Ghneim K, Haddad EK, Quakkerlaar ED, Delaloye J, Harari A, Roger T, Dunhen T, Sékaly RP, Melief CJM, Calandra T, Sallusto F, Lanzavecchia A, Wagner R, Pantaleo G, Esteban M . Based on the...
-
Dynamics of Salmonella small RNA expression in non-growing bacteria located inside eukaryotic cells
RNA Biology Ortega A, Gonzalo-Asensio J, García-Del Portillo F . Small non-coding regulatory RNAs (sRNAs) have been studied in many bacterial pathogens during infection. However, few studies have focused on how intracellular pathogens modulate sRNA...
-
The IHF regulon of exponentially growing Pseudomonas putida cells
Environmental Microbiology Silva-Rocha R, Chavarría M, Kleijn RJ, Sauer U, de Lorenzo V . Integration host factor (IHF) sites are largely absent from intergenic regions of ORFs encoding central metabolic functions in Pseudomonas putida mt-2. To gain an...