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Tyr³²³-dependent p38 activation is associated with rheumatoid arthritis and correlates with disease activity
Arthritis & Rheumatism López-Santalla M, Salvador-Bernáldez M, González-Alvaro I, Castañeda S, Ortiz AM, García-García MI, Kremer L , Roncal F, Mulero J, Martínez-A C & Salvador JM . The p38 MAPK is important in the pathogenic immune response in...
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Engineering multiple genomic deletions in Gram-negative bacteria: analysis of the multi-resistant antibiotic profile of Pseudomonas putida KT2440
Environmental Microbiology Martínez-García E & de Lorenzo V . The genome of the soil bacterium Pseudomonas putida strain KT2440 has been erased of various determinants of resistance to antibiotics encoded in its extant chromosome. To this end, we...
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Beautiful is not (necessarily) right: Overcoming the Phryne's trial syndrome
BioEssays de Lorenzo V . We live in times where image is everything. Today, a 2 year-old child has seen orders of magnitude more images during his short life than the founders of modern Science (Galileo, Newton, Mendel etc .) could have observed during...
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Dihydrosphingomyelin impairs HIV-1 infection by rigidifying liquid-ordered membrane domains
Chemistry & Biology Vieira CR, Munoz-Olaya JM, Sot J, Jiménez-Baranda S, Izquierdo-Useros N, Abad JL, Apellániz B, Delgado R, Martinez-Picado J, Alonso A, Casas J, Nieva JL, Fabriás G, Mañes S , Goñi FM. The lateral organization of lipids in cell...
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Immobilized Metal Affinity Chromatography/Reversed-Phase Enrichment of Phosphopeptides and Analysis by CID/ETD Tandem Mass Spectrometry
Methods in Molecular Biology Navajas R, Paradela A & Albar JP . Major difficulties in phosphoprotein analysis relate to the presence of a huge number of nonphosphorylated proteins and to the wide concentration dynamic range among them. In order to...
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Self-Protection and Survival of Arbovirus-Infected Mosquito Cells
Cellular Microbiology López-Montero N & Risco C . Arboviruses are serious pathogens for men but cause little damage to their arthropod vectors. We have studied how a mosquito cell line derived from one of the relevant vectors for arboviruses responds...
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The cortactin-binding domain of WIP is essential for podosome formation and extracellular matrix degradation by murine dendritic cells
European Journal of Cell Biology Bañón-Rodríguez I, Monypenny J, Ragazzini C, Franco A, Calle Y, Jones GE & Antón IM . In immature dendritic cells (DCs) podosomes form and turn over behind the leading edge of migrating cells. The Arp2/3 complex...
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Relevance of proteomics standards for the ProteoRed Spanish organization
Journal of Proteomics Martinez-Bartolome S, Blanco F & Albar JP . ProteoRed , a Spanish Initiative in the field of proteomics, is reaching international standards. Here we present ProteoRed as an example of collaborative initiative in the field of...
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Environmental biosafety in the age of Synthetic Biology: Do we really need a radical new approach?
Bioessays de Lorenzo V . The term Genetic Engineering constitutes a strong metaphor that evokes the action of joining together otherwise separate components with given properties following a rational blueprint; but this time using genes as the building...
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Constraints to virus infection in Nicotiana benthamiana plants transformed with a potyvirus amplicon
BMC Plant Biology Calvo M, Dujovny G, Lucini C, Ortuño J, Alamillo JM, Simón-Mateo C , López-Moya JJ & García JA . BACKGROUND: Plant genomes have been transformed with full-length cDNA copies of viral genomes, giving rise to what has been called...
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Emerging complexity in reactive oxygen species production and signaling during the response of plants to pathogens
Plant Physiology Vellosillo T, Vicente J, Kulasekaran S, Hamberg M & Castresana C . Plants have evolved a complex immune system to perceive microbial pathogens and respond by producing defense compounds preventing infection. Defense hormones such as...
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Septins from the phytopathogenic fungus Ustilago maydis are required for proper morphogenesis but dispensable for virulence
PLoS ONE Alvarez-Tabarés I & Pérez-Martín J . BACKGROUND: Septins are a highly conserved family of GTP-binding proteins involved in multiple cellular functions, including cell division and morphogenesis. Studies of septins in fungal cells underpin a...
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Role of Escherichia coli FtsN protein in the assembly and stability of the cell division ring
Molecular Microbiology Rico AI, García-Ovalle M, Palacios P, Casanova M & Vicente M . Deprivation of FtsN, the last protein in the hierarchy of divisome assembly , causes the disassembly of other elements from the division ring, even extending to...
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Plasmid pSM19035, a model to study stable maintenance in Firmicutes
Plasmid Lioy VS, Pratto F, de la Hoz AB, Ayora S & Alonso JC . pSM19035 is a low-copy-number theta-replicating plasmid, which belongs to the Inc18 family. Plasmids of this family, which show a modular organization, are functional in evolutionarily...
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Arabidopsis thaliana as a model for the study of plant-virus co-evolution
Philos Trans R Soc Lond B Biol Sci Pagán I, Fraile A , Fernandez-Fueyo E, Montes N, Alonso-Blanco C & García-Arenal F. Understanding plant-virus coevolution requires wild systems in which there is no human manipulation of either host or virus. To...
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Increasing omega-3 desaturase expression in tomato results in altered aroma profile and enhanced resistance to cold stress
Plant Physiology Domínguez T, Hernández ML, Pennycooke JC, Jiménez P, Martínez-Rivas JM, Sanz C, Stockinger EJ, Sánchez-Serrano JJ & Sanmartín M. One of the drawbacks in improving the aroma properties of tomato ( Solanum lycopersicum ) fruit is the...
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Three-dimensional structure of Rubella virus factories
Virology Fontana J, López-Iglesias C, Tzeng WP, Frey TK, Fernández JJ & Risco C . Viral factories are complex structures in the infected cell where viruses compartmentalize their life cycle. Rubella virus (RUBV) assembles factories by recruitment of...
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A central regulatory system largely controls transcriptional activation and repression responses to phosphate starvation in Arabidopsis
PLoS Genetics Bustos R, Castrillo G, Linhares F, Puga MI, Rubio V , Pérez-Pérez J, Solano R , Leyva A & Paz-Ares J . Plants respond to different stresses by inducing or repressing transcription of partially overlapping sets of genes. In Arabidopsis,...
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Noise and robustness in prokaryotic regulatory networks
Annual Review of Microbiology Silva-Rocha R & de Lorenzo V . Robustness is the quality of any relational object (biological or otherwise) to maintain its components, its structure, and its function despite both external changes and endogenous...
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Sirtuin 1 regulation of developmental genes during differentiation of stem cells
PNAS Calvanese V, Lara E, Suárez-Alvarez B, Abu Dawud R, Vázquez-Chantada M, Martínez-Chantar ML, Embade N, López-Nieva P, Horrillo A, Hmadcha A, Soria B, Piazzolla D, Herranz D, Serrano M, Mato JM, Andrews PW, López-Larrea C, Esteller M & Fraga MF....