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A candidate HIV/AIDS vaccine (MVA-B) lacking vaccinia virus gene C6L enhances memory HIV-1-specific T-cell responses
PLoS ONE García-Arriaza J, Nájera JL, Gómez CE, Tewabe N, Sorzano CO, Calandra T, Roger T, Esteban M . The vaccinia virus (VACV) C6 protein has sequence similarities with the poxvirus family Pox_A46, involved in regulation of host immune responses, but...
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The interplay of the EIIA(Ntr) component of the nitrogen-related phosphotransferase system (PTS(Ntr)) of Pseudomonas putida with pyruvate dehydrogenase
Biochimica et Biophysica Acta Pflüger-Grau K, Chavarría M, de Lorenzo V . Pseudomonas putida KT2440 is endowed with a variant of the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS(Ntr)), which is not related to sugar transport but...
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Severe acute respiratory syndrome coronavirus envelope protein regulates cell stress response and apoptosis
PLoS Pathogens Dediego ML, Nieto-Torres JL, Jiménez-Guardeño JM, Regla-Nava JA, Alvarez E, Oliveros JC, Zhao J, Fett C, Perlman S, Enjuanes L . Severe acute respiratory syndrome virus (SARS-CoV) that lacks the envelope (E) gene (rSARS-CoV-ΔE) is...
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Virus infection rapidly activates the P58(IPK) pathway, delaying peak kinase activation to enhance viral replication
Virology Goodman AG, Tanner BC, Chang ST, Esteban M, Katze MG. Previously we showed that the cellular protein P58(IPK) contributes to viral protein synthesis by decreasing the activity of the anti-viral protein, PKR. Here, we constructed a mathematical...
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Gene N proximal and distal RNA motifs regulate coronavirus nucleocapsid mRNA transcription
The Journal of Virology Mateos-Gómez PA, Zuñiga S, Palacio L, Enjuanes L, Sola I. Coronavirus subgenomic mRNA (sgmRNA) transcription requires a discontinuous RNA synthesis mechanism driven by the transcription-regulating sequences (TRSs), located at...
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Double-strand break repair in bacteria: a view from Bacillus subtilis
FEMS Microbiology Reviews Ayora S, Carrasco B, Cárdenas PP, César CE, Cañas C, Yadav T, Marchisone C, Alonso JC. In all living organisms, the response to double-strand breaks (DSBs) is critical for the maintenance of chromosome integrity. Homologous...
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Association of ActA to Peptidoglycan Revealed by Cell Wall Proteomics of Intracellular Listeria monocytogenes
The Journal of Biological Chemistry García-Del Portillo F , Calvo E, D'Orazio V, Pucciarelli MG. Listeria monocytogenes is a Gram-positive intracellular bacterial pathogen that colonizes the cytosol of eukaryotic cells. Recent transcriptomic studies...
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Ethylene is involved in pistil fate by modulating the onset of ovule senescence and the GA-mediated fruit set in Arabidopsis
BMC Plant Biology Carbonell-Bejerano P, Urbez C, Granell A, Carbonell J, Perez-Amador MA. Ovule lifespan is an important factor in determining the ability to set fruits and produce seeds. Once ovule senescence is established, fruit set capacity in...
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Functional analysis of the integration host factor site of the σ(54) Pu promoter of Pseudomonas putida by in vivo UV imprinting
Molecular Microbiology Valls M, Silva-Rocha R, Cases I, Muñoz A, de Lorenzo V. The integration host factor (IHF) of Pseudomonas putida connects cell growth to transcriptional activity of distinct promoters. The IHF site of the σ 54 promoter Pu of the...
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Genetic polymorphisms among C57BL/6 mouse inbred strains
Transgenic Reserach Zurita E, Chagoyen M, Cantero M, Alonso R, González-Neira A, López-Jiménez A, López-Moreno JA, Landel CP, Benítez J, Pazos F, Montoliu L. Mice from the inbred C57BL/6 strain have been commonly used for the generation and analysis of...
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The K+ uptake regulator TrkA controls membrane potential, pH homeostasis and multidrug susceptibility in Mycobacterium smegmatis
Journal of Antimicrobial Chemotherapy Castañeda-García A, Do TT, Blázquez J. Rifampicin is an important first-line antibiotic for the treatment of mycobacterial infections. Although most rifampicin-resistant strains arise through mutations in the rpoB...
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Beyond serial passages: new methods for predicting the emergence of resistance to novel antibiotics
Current Opinion in Pharmacology Martínez JL , Baquero F, Andersson DI. Market launching of a new antibiotic requires knowing in advance its benefits and possible risks, and among them how rapidly resistance will emerge and spread among bacterial...
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Control of flowering and storage organ formation in potato by FLOWERING LOCUS T
Nature Navarro C, Abelenda JA, Cruz-Oró E, Cuéllar CA, Tamaki S, Silva J, Shimamoto K, Prat S . Seasonal fluctuations in day length regulate important aspects of plant development such as the flowering transition or, in potato ( Solanum tuberosum ),...
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The DNA damage response signaling cascade regulates Proliferation of the phytopathogenic fungus Ustilago maydis in planta
The Plant Cell de Sena-Tomás C, Fernández-Álvarez A, Holloman WK, Pérez-Martín J . In the phytopathogenic fungus Ustilago maydis , the dikaryotic state dominates the period of growth occurring during the infectious phase. Dikaryons are cells in which...
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Evidence of p38γ and p38δ involvement in cell transformation processes
Carcinogenesis Cerezo-Guisado MI, del Reino P, Remy G, Kuma Y, Arthur JS, Gallego-Ortega D, Cuenda A . The p38 mitogen-activated protein kinase (p38MAPK) signal transduction pathway is an important regulator of cell processes, whose deregulation leads...
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Dikaryotic cell cycle in the phytopathogenic fungus Ustilago maydis is controlled by the DNA damage response cascade
Plant Signaling & Behavior Pérez-Martín J , de Sena-Tomás C. In a large group of fungi, mating results in a dikaryon, a cell in which the two nuclei -one from each parent cell- share a single cytoplasm for a period of time without undergoing nuclear...
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The Fimbrial Usher FimD Follows the SurA-BamB Pathway for Its Assembly in the Outer Membrane of Escherichia coli
The Journal of Bacteriology Palomino C, Marín E & Fernández LA . Fimbrial ushers are the largest β-barrel outer membrane proteins (OMPs) known to date, which function in the polymerization of fimbriae and their translocation to the bacterial surface....
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Direct injection of functional single-domain antibodies from E. coli into human cells
PLoS One. 2010; 5(12):e15227 Blanco-Toribio A, Muyldermans S, Frankel G, Fernández LÁ . Intracellular proteins have a great potential as targets for therapeutic antibodies (Abs) but the plasma membrane prevents access to these antigens. Ab fragments...
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Immunogenic profiling in mice of a HIV/AIDS vaccine candidate (MVA-B) expressing four HIV-1 antigens and potentiation by specific gene deletions
PLoS ONE García-Arriaza J, Nájera JL, Gómez CE, Sorzano CO & Esteban M . The immune parameters of HIV/AIDS vaccine candidates that might be relevant in protection against HIV-1 infection are still undefined. The highly attenuated poxvirus strain MVA is...
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Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors
The Plant Journal Godoy M, Franco-Zorrilla JM, Pérez-Pérez J, Oliveros JC, Lorenzo O & Solano R . Transcriptional regulation depends on the specificity of transcription factors (TFs) recognizing cis regulatory sequences in the promoters of target...