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An insulator embedded in the chicken {alpha}-globin locus regulates chromatin domain configuration and differential gene expression
Nucleic Acids Research Furlan-Magaril M, Rebollar E, Guerrero G, Fernández A, Moltó E, González-Buendía E, Cantero M, Montoliu L & Recillas-Targa F. Genome organization into transcriptionally active domains denotes one of the first levels of gene...
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An alternative pathway for ureide usage in legumes: enzymatic formation of a ureidoglycolate adduct in Cicer arietinum and Phaseolus vulgaris
Journal of Experimental Botany Muñoz A, Bannenberg GL, Montero O, Cabello-Díaz JM, Piedras P & Pineda M. Ureidoglycolate is an intermediate in the degradation of the ureides, allantoin and allantoate, found in many organisms. In some leguminous plant...
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ERK5 pathway regulates the phosphorylation of tumour suppressor hDlg during mitosis
Biochemical and Biophysical Research Communications Iñesta-Vaquera FA, Campbell DG, Arthur JS & Cuenda A . Human disc-large (hDlg) is a scaffold protein critical for the maintenance of cell polarity and adhesion . hDlg is thought to be a tumour...
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Functional specialization within the Vacuolar Sorting Receptor family: VSR1, VSR3 and VSR4 sort vacuolar storage cargo in seeds and vegetative tissues
The Plant Journal Zouhar J, Muñoz A & Rojo E . Two different gene families have been proposed to act as sorting receptors for vacuolar storage cargo in plants: the vacuolar sorting receptors (VSRs) and the receptor homology-transmembrane-RING H2 domain...
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Immunogenic Profiling in Mice of a HIV/AIDS Vaccine Candidate (MVA-B) Expressing Four HIV-1 Antigens and Potentiation by Specific Gene Deletions
PLoS ONE García-Arriaza J, Nájera JL, Gómez CE, Sorzano CO & Esteban M . The immune parameters of HIV/AIDS vaccine candidates that might be relevant in protection against HIV-1 infection are still undefined. The highly attenuated poxvirus strain MVA is...
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A MATE-family efflux pump rescues the Escherichia coli 8-oxoguanine-repair-deficient mutator phenotype and protects against H(2)O(2) killing
PLoS Genetics Guelfo JR, Rodríguez-Rojas A, Matic I & Blázquez J . Hypermutation may accelerate bacterial evolution in the short-term. In the long-term, however, hypermutators (cells with an increased rate of mutation) can be expected to be at a...
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Insertion of vaccinia virus C7L host range gene into NYVAC-B genome potentiates immune responses against HIV-1 antigens
PLoS ONE Nájera JL, Gómez CE, García-Arriaza J, Sorzano CO & Esteban M . The highly attenuated vaccinia virus strain NYVAC expressing HIV-1 components has been evaluated as a vaccine candidate in preclinical and clinical trials with encouraging...
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A comparative analysis of the biochemical and functional properties of C-terminal domains of autotransporters
Journal of Bacteriology Marín E, Bodelón G & Fernández LA . Autotransporters (ATs) are the largest group of proteins secreted by Gram-negative bacteria and include many virulence factors from human pathogens . ATs are synthesized as large precursors...
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Electron microscopy analysis of mammalian phosphofructokinase reveals an unusual 3-dimensional structure with significant implications for enzyme function
The FASEB Journal Arechaga I, Martínez-Costa OH, Ferreras C, Carrascosa JL & Aragón JJ. Phosphofructokinase is a sophisticated allosteric enzyme that is fundamental for the control of glycolysis . The structure of the bacterial enzyme is well...
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Immunolocalization of proteins in plants
Methods in Molecular Biology Sauer M & Friml J. Rapid advances in the field of plant biology, especially in plant cell biology, have created the need for methods that allow the localization of proteins in situ at subcellular resolution. Although in...
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Identification of Cellular Genes Induced in Human Cells After Activation of the OAS/RNaseL Pathway by Vaccinia Virus Recombinants Expressing These Antiviral Enzymes
Journal of Interferon & Cytokine Research Domingo-Gil E, González JM & Esteban M . Interferon (IFN) type I induces the expression of antiviral proteins such as 2',5'-oligoadenylate synthetases (OAS). The enzyme OAS is activated by dsRNA to produce...
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Frequency of spontaneous resistance to fosfomycin combined with different antibiotics in Pseudomonas aeruginosa
Antimicrobial Agents and Chemotherapy Rodríguez-Rojas A, Couce A & Blázquez J . Chronic infections caused by Pseudomonas aeruginosa are probably the most recurrent clinical situation where antibiotic treatment fails. The presence of hypermutable...
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Specialized pro-resolving lipid mediators in the inflammatory response: An update
Biochim Biophys Acta Bannenberg G & Serhan CN. A new genus of specialized pro-resolving mediators (SPM) which include several families of distinct local mediators (lipoxins, resolvins, protectins, and maresins) are actively involved in the clearance...
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Protein Information and Knowledge Extractor: Discovering biological information from proteomics data
Proteomics Medina-Aunon JA, Paradela A, Macht M, Thiele H, Corthals G & Albar JP . One of the main goals in proteomics is to solve biological and molecular questions regarding a set of identified proteins. In order to achieve this goal, one has to...
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The T=1 capsid protein of Penicillium chrysogenum virus is formed by a repeated helix-rich core indicative of gene duplication
Journal of Virology Luque D, González JM, Garriga D, Ghabrial SA, Havens WM, Trus B, Verdaguer N, Carrascosa JL & Castón JR . Penicillium chrysogenum virus (PcV), a member of the Chrysoviridae family, is a double-stranded RNA (dsRNA) fungal virus with...
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Massive translational repression of gene expression during mouse erythroid differentiation
Biochimica et Biophysica Acta Pradet-Balade B, Leberbauer C, Schweifer N, Boulmé F. We took advantage of a mouse erythroid differentiation system to determine the relative contribution of transcriptional and translational control during this process....
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Structural and functional characterisation of an influenza virus RNA polymerase-genomic RNA complex
Journal of Virology Resa-Infante P, Recuero-Checa MA, Zamarreño N, Llorca O & Ortín J . Replication and transcription of influenza A virus is carried out by ribonucleoproteins (RNPs) containing each genomic RNA segment associated to nucleoprotein...
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Selective induction of host genes by MVA-B, a candidate vaccine against HIV / AIDS
Journal of Virology Guerra S, González JM, Climent N, Reyburn H, López-Fernández LA, Nájera JL, Gómez CE, García F, Gatell JM, Gallart T & Esteban M . The aim of this study was to define the effects on antigen-presenting cells of the expression of HIV...
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Genetic determinants involved in the susceptibility of Pseudomonas aeruginosa to {beta}-lactam antibiotics
Antimicrobial Agents and Chemotherapy Alvarez-Ortega C, Wiegand I, Olivares J, Hancock RE & Martínez JL . The resistome of P. aeruginosa for three beta-lactam antibiotics, namely ceftazidime, imipenem and meropenem was deciphered by screening a...
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Conformational rearrangements of SV40 large T antigen during early replication events
Journal of Molecular Biology Cuesta I, Núñez-Ramírez R, Scheres SH, Gai D, Chen XS, Fanning E & Carazo JM The Simian virus 40 (SV40) large tumor antigen (LTag) functions as the replicative helicase and initiator for viral DNA replication. For SV40...