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Inhibition of NF-κB-mediated inflammation in severe acute respiratory syndrome coronavirus-infected mice increases survival
J Virol. 2014; 88(2): 913-924. Dediego ML, Nieto-Torres JL, Regla-Nava JA, Jimenez-Guardeño JM, Fernandez-Delgado R, Fett C, Castaño-Rodriguez C, Perlman S, Enjuanes L . Severe acute respiratory syndrome coronavirus (SARS-CoV) is the etiological agent...
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Dormant intracellular Salmonella enterica serovar Typhimurium discriminates among Salmonella pathogenicity island 2 effectors to persist inside fibroblasts
Infect Immun. 2014; 82(1): 221-232. Núñez-Hernández C, Alonso A, Pucciarelli MG, Casadesús J, García-Del Portillo F . Salmonella enterica uses effector proteins delivered by type III secretion systems (TTSS) to colonize eukaryotic cells. Recent in vivo...
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Temporal regulation of the Mus81-Mms4 endonuclease ensures cell survival under conditions of DNA damage
Nucleic Acids Res. 2013 Oct;41(19):8943-58. Saugar I, Vázquez MV, Gallo-Fernández M, Ortiz-Bazán MÁ, Segurado M, Calzada A , Tercero JA. The structure-specific Mus81-Eme1/Mms4 endonuclease contributes importantly to DNA repair and genome integrity...
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DNA double strand break end-processing and RecA induce RecN expression levels in Bacillus subtilis
DNA Repair (Amst). 2013 Dec 25. pii: S1568-7864(13)00288-7. Cardenas PP, Gándara C, Alonso JC . Bacillus subtilis cells respond to double strand breaks (DSBs) with an ordered recruitment of repair proteins to the site lesion, being RecN one of the...
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Uncoating of common cold virus is preceded by RNA switching as determined by X-ray and cryo-EM analyses of the subviral A-particle
Proc Natl Acad Sci USA. 2013; 110(50): 20063-20068. Pickl-Herk A1, Luque D, Vives-Adrián L, Querol-Audí J, Garriga D, Trus BL, Verdaguer N, Blaas D, Castón JR . During infection, viruses undergo conformational changes that lead to delivery of their...
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Bacillus subtilis RecA and its accessory factors, RecF, RecO, RecR and RecX, are required for spore resistance to DNA double-strand break
Nucleic Acids Res. 2014; 42(4): 2295-2307. Vlasic I, Mertens R, Seco EM, Carrasco B, Ayora S , Reitz G, Commichau FM, Alonso JC , Moeller R. Bacillus subtilis RecA is important for spore resistance to DNA damage, even though spores contain a single...
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Growth hormone prevents the development of autoimmune diabetes
Proc Natl Acad Sci USA. 2013 Nov 11. Villares R, Kakabadse D, Juarranz Y, Gomariz RP, Martínez-A C , Mellado M . Evidence supports a relationship between the neuroendocrine and the immune systems. Data from mice that overexpress or are deficient in...
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New vaccinia virus promoter as potential candidate for future vaccines
J Gen Virol. 2013 Sep 28 Di Pilato M, Mejías-Pérez E, Gómez CE, Perdiguero B, Sánchez-Sorzano CO, Esteban M . Here we describe the design and strength of a new synthetic Late-Early Optimized (LEO) vaccinia virus (VACV) promoter used as transcriptional...
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Development of magnetic nanoparticles for cancer gene therapy: a comprehensive review
ISRN Nanomaterials. 2013; ID646284 Mulens V, Morales MP, Barber DF . Since they were first proposed as nonviral transfection agents for their gene-carrying capacity, magnetic nanoparticles have been studied thoroughly, both in vitro and in vivo . Great...
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Crystallization of the C-terminal head domain of the fibre protein from a siadenovirus, turkey adenovirus 3
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013; 69(Pt 10):1135-1139 Singh AK, Ballmann MZ, Benkő M, Harrach B, van Raaij MJ . Turkey adenovirus 3 belongs to the genus Siadenovirus. Its predicted fibre protein consists of an N-terminal...
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Proteomics identifies ubiquitin-proteasome targets and new roles for chromatin-remodeling in the Arabidopsis response to phosphate starvation
J Proteomics. 2013; 94C:1-22 Iglesias J, Trigueros M, Rojas-Triana M, Fernández M, Albar JP , Bustos R, Paz-Ares J , Rubio V. . In order to identify new regulators of the phosphate (Pi) starvation signaling pathway in plants, we analyzed variation in...
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Transcriptional characterisation of the negative effect exerted by a deficiency in type II signal peptidase on extracellular protein secretion in Streptomyces lividans
Appl Microbiol Biotechnol. 2013 Sep 26 Gullón S, Arranz EI, Mellado RP . Bacterial lipoproteins are a specialised class of membrane proteins that represent a small percentage of the proteome of Gram-positive bacteria, yet these lipoproteins have been...
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Bacterial division proteins FtsZ and ZipA induce vesicle shrinkage and cell membrane invagination
J Biol Chem. 2013; 288(37):26625-34 Cabré EJ, Sánchez-Gorostiaga A, Carrara P, Ropero N, Casanova M, Palacios P, Stano P, Jiménez M, Rivas G, Vicente M . Permeable vesicles containing the proto-ring anchoring ZipA protein shrink when FtsZ, the main...
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Deletion of the vaccinia virus gene A46R, encoding for an inhibitor of TLR signalling, is an effective approach to enhance the immunogenicity in mice of the HIV/AIDS vaccine candidate NYVAC-C
PLoS One. 2013; 8(9):e74831 Perdiguero B, Gómez CE, Di Pilato M, Sorzano CO, Delaloye J, Roger T, Calandra T, Pantaleo G, Esteban M . Viruses have developed strategies to counteract signalling through Toll-like receptors (TLRs) that are involved in the...
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Disposable amperometric magnetoimmunosensors using nanobodies as biorecognition element. Determination of fibrinogen in plasma
Biosens Bioelectron. 2013;52C:255-260 Campuzano S, Salema V, Moreno-Guzmán M, Gamella M, Yáñez-Sedeño P, Fernández LA , Pingarrón JM. Two different fibrinogen (Fib) amperometric immunosensing designs based on the use of magnetic beads (MBs) and a novel...
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Arabidopsis semidwarfs evolved from independent mutations in GA20ox1, ortholog to green revolution dwarf alleles in rice and barley
PNAS. 2013;110(39):15818-23 Barboza L, Effgen S, Alonso-Blanco C , Kooke R, Keurentjes JJ, Koornneef M, Alcázar R. Understanding the genetic bases of natural variation for developmental and stress-related traits is a major goal of current plant...
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Selection of single domain antibodies from immune libraries displayed on the surface of E. coli cells with two β-domains of opposite topologies
PLoS ONE 8(9): e75126 Salema V, Marín E, Martínez-Arteaga R, Ruano-Gallego D, Fraile S, Margolles Y, Teira X, Gutierrez C, Bodelón G, Fernández LA . Screening of antibody (Ab) libraries by direct display on the surface of E. coli cells is hampered by...
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Mapping cytoskeletal protein function in cells by means of nanobodies
Cytoskeleton (Hoboken). 2013 Jul 2 Van Audenhove I, Van Impe K, Ruano-Gallego D, De Clercq S, De Muynck K, Vanloo B, Verstraete H, Fernández LA , Gettemans J. Nanobodies or VHHs are single domain antigen binding fragments derived from heavy-chain...
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Engineering a replication-competent, propagation-defective middle East respiratory syndrome coronavirus as a vaccine candidate
MBio. 2013 Sep 10;4(5). pii: e00650-13 Almazán F, Dediego ML, Sola I, Zuñiga S, Nieto-Torres JL, Marquez-Jurado S, Andrés G, Enjuanes L . Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging coronavirus infecting humans that is...
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Specialized functions of the PP2A subfamily II catalytic subunits PP2A-C3 and PP2A-C4 in the distribution of auxin fluxes and development in Arabidopsis
Plant J. 2013 Mar;73(5):862-72 Ballesteros I, Domínguez T, Sauer M, Paredes P, Duprat A, Rojo E , Sanmartín M, Sánchez-Serrano JJ . Protein phosphorylation is a key molecular switch used to transmit information in biological signalling networks. The...