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Regulation of DNA methylation patterns by CK2-mediated phosphorylation of Dnmt3a
Cell Rep. 2014; pii: S2211-1247(14)00532-4. Deplus R, Blanchon L, Rajavelu A, Boukaba A, Defrance M, Luciani J, Rothé F, Dedeurwaerder S, Denis H, Brinkman AB, Simmer F, Müller F, Bertin B, Berdasco M, Putmans P, Calonne E, Litchfield DW, de Launoit Y,...
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Programming controlled adhesion of E. coli to target surfaces, cells, and tumors with synthetic adhesins
ACS Synth Biol. 2014; doi: 10.1021/sb500252a. Piñero-Lambea C, Bodelón G, Fernández-Periáñez R, Cuesta AM, Alvarez-Vallina L, Fernández LA . In this work we report synthetic adhesins (SAs) enabling the rational design of the adhesion properties of E....
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Molecular characterization of a lizard adenovirus reveals the first atadenovirus with two fiber genes, and the first adenovirus with either one short or three long fibers per penton
J Virol. 2014; pii: JVI.00306-14. Pénzes JJ, Menéndez-Conejero R, Condezo GN, Ball I, Papp T, Doszpoly A, Paradela A , Pérez-Berná AJ, López-Sanz M, Nguyen TH, van Raaij MJ , Marschang RE, Harrach B, Benkő M, San Martín C . Although adenoviruses have...
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Rapid fluorescent reporter quantification by leaf disc analysis and its application in plant-virus studies
Plant Methods. 2014; 10: 22. Pasin F, Kulasekaran S, Natale P, Simón-Mateo C , García JA . Background : Fluorescent proteins are extraordinary tools for biology studies due to their versatility; they are used extensively to improve comprehension of...
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Phosphatidylinositol 4,5-bisphosphate triggers activation of focal adhesion kinase by inducing clustering and conformational changes
Proc Natl Acad Sci U S A. 2014;. pii: 201317022. Goñi GM, Epifano C, Boskovic J, Camacho-Artacho M, Zhou J, Bronowska A, Martín MT, Eck MJ, Kremer L , Gräter F, Gervasio FL, Perez-Moreno M, Lietha D. Focal adhesion kinase (FAK) is a nonreceptor...
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Deletion of the membrane protein Lmo0412 increases the virulence of Listeria monocytogenes
Microbes Infect. 2014 Jul 19. pii: S1286-4579(14)00089-6. Quereda JJ , Pucciarelli MG. Listeria monocytogenes is a facultative intracellular pathogen that causes gastroenteritis, meningitis, encephalitis and maternofetal infections. 20–30% of...
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Response to copper of Acidithiobacillus ferrooxidans ATCC 23270 grown in elemental sulfur
Res Microbiol. 2014; pii: S0923-2508(14)00107-7. Almárcegui RJ, Navarro CA, Paradela A , Albar JP , von Bernath D, Jerez CA. The response of Acidithiobacillus ferrooxidans ATCC 23270 to copper was analyzed in sulfur-grown cells by using quantitative...
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Application of yeast-two hybrid assay to chemical genomic screens: a high-throughput system to identify novel molecules modulating plant hormone receptor complexes
Methods Mol Biol. 2014; 1056 :35-43. Chini A . Phytohormones are endogenous signalling molecules that regulate plant development, adaptation to the environment, and survival. Upon internal or external stimuli, hormones are quickly accumulated and...
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A Blk-p190RhoGAP signalling module downstream of activated Gα13 functionally opposes CXCL12-stimulated RhoA activation and cell invasion
Cell Signal. 2014;. pii: S0898-6568(14)00227-7. Bartolomé RA, Díaz-Martínez M, Coló GP, Arellano-Sánchez N, Torres-Ayuso P, Kleinovink JW, Mérida I , Teixidó J. Activation of the GTPase RhoA linked to cell invasion can be tightly regulated following Gα...
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Virus-induced gene silencing in transgenic plants: transgene silencing and reactivation associate with two patterns of transgene body methylation
Plant J. 2014; 79 (3): 440-452. Zhao M, San León D, Delgadillo MO, García JA , Simón-Mateo C . We used bisulfite sequencing to study the methylation of a viral transgene whose expression was silenced upon plum pox virus infection of the transgenic...
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Experimental evolution of an oncolytic vesicular stomatitis virus with increased selectivity for p53-deficient cells
PLoS One. 2014; 9 (7): e102365. Garijo R, Hernández-Alonso P, Rivas C , Diallo JS, Sanjuán R. Experimental evolution has been used for various biotechnological applications including protein and microbial cell engineering, but less commonly in the...
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Functional signature for the recognition of specific target mRNAs by human Staufen1 protein
Nucleic Acids Res. 2014; 42 (7): 4516-4526. de Lucas S, Oliveros JC, Chagoyen M , Ortín J . Cellular messenger RNAs (mRNAs) are associated to proteins in the form of ribonucleoprotein particles. The double-stranded RNA-binding (DRB) proteins play...
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Crystallization of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34
Acta Crystallogr F Struct Biol Commun. 2014; 70 (Pt 7): 970-975. Granell M, Namura M, Alvira S, Garcia-Doval C, Singh AK, Gutsche I, van Raaij MJ , Kanamaru S. The phage-proximal part of the long tail fibres of bacteriophage T4 consists of a trimer of...
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Rational design of a ligand-based antagonist of jasmonate perception
Nat Chem Biol. 2014; doi: 10.1038/nchembio.1575. Monte I, Hamberg M, Chini A, Gimenez-Ibanez S, García-Casado G, Porzel A, Pazos F, Boter M, Solano R . (+)-7- iso -Jasmonoyl-L-isoleucine (JA-Ile) regulates developmental and stress responses in plants....
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A unified nomenclature and amino acid numbering for human PTEN
J Virol. 2014; pii: JVI.00306-14. Pulido R, Baker SJ, Barata JT, Carracedo A, Cid VJ, Chin-Sang ID, Davé V, den Hertog J, Devreotes P, Eickholt BJ, Eng C, Furnari FB, Georgescu MM, Gericke A, Hopkins B, Jiang X, Lee SR, Lösche M, Malaney , Matias-Guiu...
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Screening of protein-protein and protein-DNA interactions using microarrays: Applications in biomedicine
Adv Protein Chem Struct Biol. 2014; 95: 231-281. Casado-Vela J, Fuentes M, Franco-Zorrilla JM . In this report, we focus on two different array-based technologies that enable large-scale screening of protein interactions. First, protein arrays focus on...
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3D cryo-electron reconstruction of BmrA, a bacterial multidrug ABC transporter in an inward-facing conformation and in a lipidic environment
J Mol Biol. 2014; 426 (10): 2059-2069. Fribourg PF, Chami M, Sorzano CO , Gubellini F, Marabini R, Marco S, Jault JM, Lévy D. ABC ( A TP- b inding c assette) membrane exporters are efflux transporters of a wide diversity of molecule across the membrane...
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Lineage-restricted function of the pluripotency factor NANOG in stratified epithelia
Nat Commun. 2014; 5: 4226. Piazzolla D, Palla AR, Pantoja C, Cañamero M, de Castro IP, Ortega S, Gómez-López G, Dominguez O, Megías D, Roncador G, Luque-Garcia JL, Fernandez-Tresguerres B, Fernandez AF, Fraga MF , Rodriguez-Justo M, Manzanares M,...
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Efficient initial volume determination from electron microscopy images of single particles
Bioinformatics. 2014; pii: btu404. Vargas J, Alvarez-Cabrera AL, Marabini R, Carazo JM , Sorzano CO. Motivation: Structural information of macromolecular complexes provides key insights into the way they carry out their biological functions. The...
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Altered microRNA expression after infection with human cytomegalovirus leads to TIMP3 downregulation and increased shedding of metalloprotease substrates, including MICA
J Immunol. 2014; pii: 1303441. Esteso G, Luzón E, Sarmiento E, Gómez-Caro R, Steinle A, Murphy G, Carbone J, Valés-Gómez M , Reyburn HT . Proteolytic shedding of ligands for the NK group 2D (NKG2D) receptor is a strategy used by tumors to modulate...