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Conversion of the OmpF Porin into a Device to Gather Amyloids on the E. coli Outer Membrane
ACS Synth Biol. 2021 Dec 1. S Vendrell-Fernández, P Lozano-Picazo, P Cuadros-Sánchez, MM Tejero-Ojeda, R Giraldo Abstract Protein amyloids are ubiquitous in natural environments. They typically originate from microbial secretions or spillages from...
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An evolutionarily ancient fatty acid desaturase is required for the synthesis of hexadecatrienoic acid, which is the main source of the bioactive jasmonate in Marchantia polymorpha
New Phytol. 2021 Nov 30. G Soriano, S Kneeshaw, G Jimenez-Aleman, ÁM Zamarreño, JM Franco-Zorrilla, MF Rey-Stolle, C Barbas, JM García-Mina, R Solano Abstract Jasmonates are fatty acid-derived hormones that regulate multiple aspects of plant...
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MOV10 Helicase Interacts with Coronavirus Nucleocapsid Protein and Has Antiviral Activity
mBio. 2021 Oct 26;12(5):e0131621. L Wang, I Sola, L Enjuanes, S Zuñiga Abstract Coronaviruses (CoVs) are emergent pathogens that may cause life-threatening respiratory diseases in humans. Understanding of CoV-host interactions may help to identify...
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Efficient markerless integration of genes in the chromosome of probiotic E. coli Nissle 1917 by bacterial conjugation
Microb Biotechnol. 2021 Nov 9. Seco EM, Fernández LA Abstract The probiotic strain Escherichia coli Nissle 1917 (EcN) is a common bacterial chassis in synthetic biology developments for therapeutic applications given its long track record of safe...
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Resident macrophage-dependent immune cell scaffolds drive anti-bacterial defense in the peritoneal cavity
Immunity. 2021 Nov 9;54(11):2578-2594.e5. Vega-Pérez A, Villarrubia LH , Godio C, Gutiérrez-González A,Feo-Lucas L, Ferriz M, Martínez-Puente N, Alcaín J, Mora A, Sabio G , López-Bravo M, Ardavín C Abstract Peritoneal immune cells reside unanchored...
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Middle East respiratory syndrome coronavirus vaccine based on a propagation-defective RNA replicon elicited sterilizing immunity in mice
Proc Natl Acad Sci U S A. 2021 Oct 26;118(43):e2111075118. Gutiérrez-Álvarez J, Honrubia JM, Sanz-Bravo A, González-Miranda E, Fernández-Delgado R, Rejas MT, Zúñiga S, Sola I, Enjuanes L Abstract Self-amplifying RNA replicons are promising platforms...
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SARS-CoV-2 Fears Green: The Chlorophyll Catabolite Pheophorbide A Is a Potent Antiviral
Pharmaceuticals (Basel). 2021 Oct 15;14(10):1048. Jimenez-Aleman GH, Castro V, Londaitsbehere A, Gutierrez-Rodríguez M, Garaigorta U, Solano R, Gastaminza P Abstract SARS-CoV-2 pandemic is having devastating consequences worldwide. Although vaccination...
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Mitochondrial Stress Induces Plant Resistance Through Chromatin Changes
Front Plant Sci. 2021 Sep 22;12:704964. eCollection 2021. López Sánchez A, Hernández Luelmo S, Izquierdo Y, López B, Cascón T, Castresana C Abstract Plants respond more efficiently when confronted with previous similar stress. In the case of pathogens,...
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ssDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces
Commun Biol. 2021 Oct 7;4(1):1169. Y Al-Ramahi, Ak Nyerges, Y Margolles, L Cerdán, G Ferenc, C Pál, LA Fernández , V de Lorenzo Abstract ssDNA recombineering has been exploited to hyperdiversify genomically-encoded nanobodies displayed on the surface...
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Targetron-Assisted Delivery of Exogenous DNA Sequences into Pseudomonas putida through CRISPR-Aided Counterselection
ACS Synth Biol. 2021 Oct 2. E Velázquez , Y Al-Ramahi, J Tellechea-Luzard, N Krasnogor, V de Lorenzo Abstract Genome editing methods based on group II introns (known as targetron technology) have long been used as a gene knockout strategy in a wide...
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Cryo-EM density maps adjustment for subtraction, consensus and sharpening
J Struct Biol. 2021 Aug 29;213(4):107780. E Fernández-Giménez, M Martínez , R Sánchez-García, R Marabini, E Ramírez-Aportela, P Conesa, JM Carazo, COS Sorzano Abstract Electron cryomicroscopy (cryo-EM) has emerged as a powerful structural biology...
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Assisted assembly of bacteriophage T7 core components for genome translocation across the bacterial envelope
Proc Natl Acad Sci U S A. 2021 Aug 24;118(34):e2026719118. M Pérez-Ruiz, M Pulido-Cid, JR Luque-Ortega, JM Valpuesta, A Cuervo, JL Carrascosa Abstract In most bacteriophages, genome transport across bacterial envelopes is carried out by the tail...
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Expression of the ISPpu9 transposase of Pseudomonas putida KT2440 is regulated by two small RNAs and the secondary structure of the mRNA 5'-untranslated region
Nucleic Acids Res. 2021 Aug 11;gkab672. G Gómez-García, A Ruiz-Enamorado, L Yuste, F Rojo , R Moreno Abstract Insertion sequences (ISs) are mobile genetic elements that only carry the information required for their own transposition. Pseudomonas putida...
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Oxylipins From Different Pathways Trigger Mitochondrial Stress Signaling Through Respiratory Complex III
Front. Plant Sci., 29 July 2021 Y Izquierdo, L Muñiz, J Vicente, S Kulasekaran, V Aguilera, A López Sánchez, A Martínez-Ayala, B López, T Cascón, C Castresana Abstract Plant oxylipins are signaling molecules produced from fatty acids by oxidative...
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A small molecule antagonizes jasmonic acid perception and auxin responses in vascular and nonvascular plants
Plant Physiol. 2021 Aug 10;kiab369 A Chini, I Monte, G Fernández-Barbero, M Boter, G Hicks, N Raikhel, Roberto Solano Abstract The phytohormone jasmonoyl-L-isoleucine (JA-Ile) regulates many stress responses and developmental processes in plants. A...
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Engineering Tropism of Pseudomonas putida toward Target Surfaces through Ectopic Display of Recombinant Nanobodies
ACS Synth Biol. 2021 Aug 2. S Fraile, M Briones, M Revenga-Parra, V de Lorenzo, E Lorenzo, E Martínez-García Abstract Gram-negative bacteria are endowed with complex outer membrane (OM) structures that allow them to both interact with other organisms...
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CTP promotes efficient ParB-dependent DNA condensation by facilitating one-dimensional diffusion from par S
Elife. 2021 Jul 12;10:e67554. F de Asis Balaguer, C Aicart-Ramos, GLm Fisher, S de Bragança, EM Martin-Cuevas, CL Pastrana, MS Dillingham, F Moreno-Herrero Abstract Faithful segregation of bacterial chromosomes relies on the ParABS partitioning system...
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DeepEMhancer: a deep learning solution for cryo-EM volume post-processing
Commun Biol. 2021 Jul 15;4(1):874. R Sanchez-Garcia, JGomez-Blanco, A Cuervo, JM Carazo, COS Sorzano, J Vargas Abstract Cryo-EM maps are valuable sources of information for protein structure modeling. However, due to the loss of contrast at high...
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Tolerance to NADH/NAD + imbalance anticipates aging and anti-aging interventions
iScience 2021 Jun 8;24(7):102697 AJ Alonso-Lavin, D Bajić, JF Poyatos Abstract Redox couples coordinate cellular function, but the consequences of their imbalances are unclear. This is somewhat associated with the limitations of their experimental...
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The combined vaccination protocol of DNA/MVA expressing Zika virus structural proteins as efficient inducer of T and B cell immune responses
Emerg Microb Infect. 2021 Dec;10(1):1441-1456. P Pérez, MA Martín-Acebes, T Poderoso, A Lázaro-Frías, JC Saiz, COS Sorzano, M Esteban, J García-Arriaza Abstract Zika virus (ZIKV) is a mosquito-borne pathogen with public health importance due to the...