Antimicrob Agents Chemother. 2012 May;56(5):2767-9

Couce A, Briales A, Rodríguez-Rojas A, Costas C, Pascual A, Blázquez J.

To determine whether the overexpression of chromosomal genes can confer fosfomycin resistance, genomewide screening of a complete set of 5,272 plasmid-expressed open reading frames of Escherichia coli (ASKA collection) was performed. Major results are that

(i) no clinical level of resistance is achieved by overexpressing chromosomal genes, except murA;

(ii) this level is reached at a low fitness cost; and

(iii) this cost is much lower than that imposed by other mutations conferring fosfomycin resistance.