Nucleic Acids Res. 2022 Nov 24;gkac1059.

E Martínez-García, S Fraile, E Algar, T Aparicio, E Velázquez, Be Calles, H Tas, B Blázquez, B Martín, C Prieto, L Sánchez-Sampedro, MHH Nørholm, DC Volke, NT Wirth, P Dvořák, L Alejaldre, L Grozinger, M Crowther, A Goñi-Morenom, PI Nikel, J Nogales, V de Lorenzo

Abstract

The SEVA platform was launched one decade ago, both as a database (DB) and as a physical repository of plasmid vectors for genetic analysis and engineering of Gram-negative bacteria with a structure and nomenclature that follows a strict, fixed architecture of functional DNA segments. While the current update keeps the basic features of earlier versions, the platform has been upgraded not only with many more ready-to-use plasmids but also with features that expand the range of target species, harmonize DNA assembly methods and enable new applications. In particular, SEVA 4.0 includes (i) a sub-collection of plasmids for easing the composition of multiple DNA segments with MoClo/Golden Gate technology, (ii) vectors for Gram-positive bacteria and yeast and [iii] off-the-shelf constructs with built-in functionalities. A growing collection of plasmids that capture part of the standard-but not its entirety-has been compiled also into the DB and repository as a separate corpus (SEVAsib) because of its value as a resource for constructing and deploying phenotypes of interest. Maintenance and curation of the DB were accompanied by dedicated diffusion and communication channels that make the SEVA platform a popular resource for genetic analyses, genome editing and bioengineering of a large number of microorganisms.

doi: 10.1093/nar/gkac1059.